![]() The Novex™ Semi-Dry Blotter requires an external power supply for operation (up to 800 mA, depending on the number and size of blots). PVDF: pre-wet in methanol or ethanol (100) for 30 seconds, briefly rinse in deionized water, and equilibrate in transfer buffer for 5 minutes. For dry transfer, follow manufacturer’s instructions for preparation of membrane. Choose between Invitrolon™ PVDF ( LC2002 or LC2005), or nitrocellulose ( LC2000 or LC2001) containing sandwiches. Prepare transfer buffer for wet or semi-dry transfer based on gel chemistry. We offer precut (8.3 cm × 7.3 cm) and pre-assembled membranes and filter paper sandwiches (available separately) to further simplify and speed the western blotting process. The Invitrogen iBlot 3 Western Blot Transfer System provides fast western transfer without the need for buffers. Fast – Blotting is typically completed in 30-60 min.Durable – Stainless steel and platinum-coated titanium electrodes allow for corrosion-free operation.Flexible – Field strength across the blot is maximized and evenly distributed, for efficient and complete transfers regardless of the number and size of gels blotted.High-capacity – A large, 20 x 20 cm transfer surface allows up to two midi gels or four mini gels to be transferred at one time.Economical – Horizontal configuration requires less than 200 ml of buffer to transfer.The Novex™ Semi-Dry Blotter is designed for convenience and efficiency: The system enables blot transfer of protein in as little as 3 minutes without sacrificing performance when compared with traditional tank protein blotting. The platinum-coated titanium anode plate and stainless steel cathode plate provide fast and efficient electroblotting using less transfer buffer than wet (tank) blotting methods. The Trans-Blot Turbo Transfer System is a high-performance western blotting transfer system designed to provide rapid transfers with high efficiency. Assemble iBlot 2 Transfer Stacks on the iBlot 2 Gel Transfer. Pairing the Power Blotter Pre-cut Membranes and Filters with the Power Blotter 1-Step Transfer Buffer allow for high efficiency semi-dry transfer of proteins in. Perform blotting within 15 minutes of assembling the stacks with the gel. One of the key steps in the western blot workflow is the transfer of proteins from the polyacrylamide gel after electrophoresis to the nitrocellulose or polyvinylidene difluoride (PVDF) membrane so that specific proteins can be detected using immune-detection techniques. Wear protective gloves and safety glasses when working in a laboratory environment. Close and latch the lid of the device carefully to ensure that the Transfer Stack and electrical contacts do not shift. To ensure safe, reliable operation, always operate the NovexSemi-Dry Blotter according to instructions provided in this manual. The Novex™ Semi-Dry Blotter is designed for fast set-up and ease of use. The NovexSemi-Dry Blotter is classified as Class II for protection against electrical shock. Simultaneous transfer can be achieved from up to 2 midi gels, 4 mini gels, or 2 E-PAGE™ gels. The Novex™ Semi-Dry Blotter quickly and efficiently transfers proteins and nucleic acids from mini and midi gels to membranes.
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